Extraction And Visualisation Of The Kiwi Fruit And...

Title: Extraction and Visualisation of the Kiwi Fruit and Strawberry Fruit DNA Introduction: All organisms have DNA (Deoxyribonucleic Acid) in the nuclei of their cells. The aim of this experiment is to extract this DNA using common household detergent to breakdown the cellular membrane then through the use of ethanol to extract the DNA from the Kiwi and Strawberry fruit. After the DNA has been extracted the DNA will be visualised by agarose gel electrophoresis which is done by adding a dye to the extracted DNA then being placed in the gel, once the negatively charged DNA has been placed in the gel it will slowly move towards the positively charged end (anode) then is viewed under an ultraviolet light then compared to the control of known lengths of DNA to show the size of the unknown DNA samples. Materials Methods: The requirements for this practical are a small sample of diced kiwi fruit, detergent, zip lock bag, 1-10mL auto-pipette, chux cloth, water, funnel, conical flask, 15mL test tube, ice (in esky), texter, ethanol, small plastic hook, 1.5mL eppendorf tube, micro-centrifuge, paper towel, 10-200uL auto-pipette, distilled water, heat block capable of reaching 55⠁ °C, agarose gel, intact plasmid (pBluescript), salt, linearized plasmid (cut once), linearized plasmid (cut twice) cut with restriction enzymes, tips for both 10-200uL and the 1-10mL auto-pipettes, safety glasses, an ultra-violet light and a camera to capture the results are needed to complete the experiment.